Tomokazu Konishi
Akita Prefectural University, JapanPresentation Title:
Deficiencies and improvements in lipoprotein quantification methods
Abstract
Problems with Lipoprotein Measurement Methods: Conventional ultracentrifugation is unsuitable, as the excessive salt concentration and centrifugal force render it inadequate for protein separation. HPLC (gel filtration) enables separation under milder conditions, but data analysis presented challenges. The speaker conducted data-driven analysis, concluding that the primary component of lipoproteins is protein, not lipids; HDL quantities are lower than previously thought; and what ultracentrifugation detected were fragments of degraded LDL. Comparing patients and healthy individuals revealed that reduced HDL1 (precursor to mHDL) and increased levels of the newly identified LAC1 (a complex of plasmin inhibitor protein and cholesterol) correlate with atherosclerosis risk. Current enzymatic methods cannot measure these risk factors and are therefore meaningless for atherosclerosis risk assessment.
Improving Measurement Methods: HPLC is highly effective but costly and difficult for multi-sample processing; therefore, an electrophoresis method is under development. This is scheduled for release within two years, aiming for simplicity and multi-sample capability. Additionally, simple measurement methods like enzymatic assays are under consideration, though this remains an open question.
Prospects for Treatment: Data concerning treatment is accumulating through HPLC measurements and is scheduled for release within one to two years. It is anticipated that the widespread adoption of the electrophoresis method will increase insights.
Biography
Tomokazu Konishi completed his Ph.D. at the age of 28 years from Nagoya University, Japan. He is an informatician with a background in biochemistry.
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